Sanitation Methods for Hand-Washed Equipment
While the use of mechanical washers is recommended for enclosures, chambers, and other moveable equipment, sanitation of cages and equipment by hand with hot water and deter¬gents or disinfectants can also be effective but requires considerable atten¬tion to detail. Regular evaluation of sanitation effectiveness can be performed by evaluating processed materials using microbiologic culture or organic material detection systems (e.g., adenosine triphosphate (ATP) bioluminescence) (Guide pp. 71, 73).
Proper sanitation of animal use equipment is important to minimize the potential spread of pathogens from animal to animal and minimize animal odors/residues that may influence research outcomes (e.g., behavioral testing). Effectiveness of sanitation methods can be based on testing for the presence of ATP on surfaces (a surrogate for residual microbiological or animal material contamination). Acceptable levels are based on hospital safety standards (<300 RLU per 100 sq. cm.).
Laboratories handwashing animal use equipment should use a method proven to be effective. If research staff request a method of cleaning other than the ones we have validated below, a minimum of 2 passing results will be required.
In all cases the equipment should first be wiped/rinsed to remove gross debris.
When using a cleaning detergent, the following steps should be performed:
- Dampen your sponge, brush, towel, or equivalent with hot water, and apply a few drops of concentrated detergent directly onto the sponge or other cleaning implement.
- Disperse the soap throughout the sponge to create soapsuds.
- Scrub the surface until no visible debris remains, generally for 10 seconds or more.
- Rinse off the soap with clean water.
- Wipe surface dry or allow to air dry.
When using a chemical disinfectant, the following steps should be performed:
- Use available disinfectant to completely clean the surface of all remaining debris.
- Leave surface wet for >1min following manufacturer’s instructions.
- Wipe with a clean towel or allow to air dry. If you use 10% bleach, rinse thoroughly with clean water.
|Cleaning Detergent||Soap(such as dishwashing detergent)||Sulfuric acid, mono-C10-16-alkyl esters, sodium salts Poly(oxy-1,2-ethanediyl), alpha-sulfo-omega-hydroxy-, C10-16-alkyl ethers, sodium salts Amine oxides, C10-16-alkyldimethyl Ethanol||Dish soap SDS|
|Cleaning Detergent||Liquinox®||Sodium alkylbenzene sulfonate
|Chemical Disinfectant||EtOH >=50%||Ethanol or Isopropanol||CDC guideline|
|Chemical Disinfectant||Bleach (10% of stock solution)||Sodium hypochlorite||CDC guideline|
Ethylene Glycol Monobutyl Ether (2-Butoxyethanol) Diisobutylphenoxyethoxyethyldimethylbenzyl ammonium chloride
|CaviCide - Spray
CaviCide - Wipe
|Chemical Disinfectant||MB-10||Sodium Chlorite
Sodium Dichloroisocyanurate dihydrate
|Chemical Disinfectant||Peroxigard™||Concentrate - Hydrogen Peroxide||Peroxigard|
|Chemical Disinfectant||neodisher LaboClean FT™||Chlorine-based bleaching agents
The official version of this information will only be maintained in an on-line web format. Any and all printed copies of this material are dated as of the print date. Please make certain to review the material on-line prior to placing reliance on a dated printed version.